Determination of Bacterial Diversity of Propolis Microbiota

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Date
2023-03
Authors
Ersoy-Ömeroğlu, Esra
Arserim-Uçar, Dilhun Keriman
Yeğin, Zeynep
Çağlayan, Nevzat
Zafer-Yurt, Mediha Nur
Taşbaşı, Behiye Büşra
Acar, Elif Esma
Uçak, Samet
Özalp, Veli Cengiz
Sudağıdan, Mert
Journal Title
Journal ISSN
Volume Title
Publisher
Chemistry and Biodiversity
Abstract
Propolis is a natural resinous mixture produced by the excretions of honeybees. PCR amplification of the 16S rRNA gene region was achieved using DNA of pre-enriched propolis samples collected from Apis mellifera production hives (n=37) in Eastern Türkiye (Bingöl and its regions). Next-generation sequencing and metabarcoding techniques were used to identify bacterial communities in propolis samples. Firmicutes dominated the phylum structure, with Proteobacteria, Actinobacteria, Tenericutes, and Spirochaetes following. The top three bacterial families were Bacillaceae, Enterobacteriaceae, and Enterococcaceae. Bacillus (dominantly B. badius and B. thermolactis at the species level) was recognized at the genus level, followed by Enterococcus and Clostridium sensu stricto. Our study comprehensively identified the bacterial diversity of propolis samples. Further investigations targeting to enlighten the microbiota of propolis and its potential application fields are required to gain better insight into ecological, nutritional, and medicinal perspectives.
Description
Published by Chemistry and Biodiversity; https://doi.org/10.1002/cbdv.202201182; Esra Ersoy Omeroglu, Biology Department, Basic and Industrial Microbiology Section, Faculty of Science, Ege University, Izmir, 35040, Türkiye; Dılhun Keriman Arserim-Uçar,Nutrition and Dietetics Department, Faculty of Health Sciences, Bingöl University, Bingöl, 12000, Türkiye; Zeynep Yegin, Medical Laboratory Techniques Program, Vocational School of Health Services, Sinop University, Sinop, 57000, Türkiye; Nevzat Çağlayan, Department of Crop and Animal Production, Vocational School of Food, Agriculture, and Livestock, Beekeeping Program, Bingöl University, Bingöl, 12000, Türkiye; Mediha Nur Zafer Yurt, Behiye Busra Tasbasi, Elif Esma Acar, Mert Sudagidan, KIT-ARGEM R&D Center, Konya Food and Agriculture University, Meram, Konya, 42080, Türkiye; Samet Ucak, Department of Medical Biology and Genetics, Faculty of Medicine, Istanbul Aydın University, Istanbul, 34295, Türkiye; Veli Cengiz Ozalp, Department of Medical Biology, Faculty of Medicine, Atilim University, Ankara, 06830, Türkiye.
Keywords
Apis mellifera, metabarcoding, microbiota, next generation sequencing, propolis
Citation
http://hdl.handle.net/20.500.14411/1949
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